"histologic structure and mineral components of secondary dentin formed by endochondral bone matrix gelatin implantation in rabbit pulp cavity"

many investigators use bone matrix gelatin for bone induction but it is used rarely for repair of teeth defects. this study was designed to evaluate secondary dentin formation by endochondral bone matrix gelatin (e-bmg) in rabbit. e-bmg was prepared from tibia and femur of 4 deutsche-poland rabbits with average ages of 4-6 months. the prepared e-bmg was implanted in right incisor teeth pulps of 8 rabbits as experimental groups and left incisor teeth pulps selected as control groups. the light and scanning electron microscopic studies were performed on days 28 and 60 after operation. also, new secreted matrix was analyzed on experimental, control and normal groups. the histological results showed secondary dentin and osteodentin formation in experimental group on day 28 after operation. the scanning electron microscopic observation on day 60 after operation in experimental group showed mineralized mass on site of e-bmg implantation. in contrast, in control group no mineralized mass was shown. analyzing of new secreted matrix in experimental group showed the high deposition of calcium and phosphate on e-bmg implantation site. results of present investigation indicate that implantation of e-bmg in pulp cavity could induce secondary dentin and osteodentin formation in rabbit. e-bmg could be a suitable biomaterial for secondary dentin formation in pulp cavity.